Scientific publication (ABSTRACTS & Links)



2019 - - - - - - - - - - - - - - - - - - - - - - - - - -



Soil stabilisation by water repellency under no-till management for soils with contrasting mineralogy and carbon quality
No-till soil management is common around the globe, but the impacts on soil structural quality varies depending on cropping practice and inherent soil properties. This study explored water repellency as a driver of soil stabilization, as affected by soil mineralogy, granulometry and organic carbon quality in three Mollisols and one Vertisol under no-till management and with different levels of cropping intensity. The studied soils were located along a west-east textural gradient in the northern part of the Pampean region of Argentina. Cropping intensity treatments evaluated in each one of the soils were: Poor Agricultural Practices (PAP) close to a monoculture, Good Agricultural Practices (GAP) involving a diverse crop rotation and more targeted inputs, and the soil in the surrounding natural environment (NE) as a reference. NE had the greatest aggregate stability (MWD) of all cropping intensities, with GAP being more stable than PAP for Mollisols and PAP being greater than GAP for the Vertisol. This trend matched the Repellency Index (Rindex), with greater Rindex associated with greater MWD, including the difference between the Mollisols and Vertisol. However, the persistence of water repellency, measured by the Water Drop Penetration Time (WDPT) test followed the trend NE > GAP > PAP regardless of soil type. The increases in Rindex and MWD were related to higher intensification as measured by the Crop Sequence Index, and decreased with greater soybean occurrence in the sequence. Both WDPT and Rindex were closely related to aggregate stability, particularly for Mollisols. These results highlight the importance of considering the inherent soil characteristics texture and mineralogy to understand aggregate stabilization mediated by water repellency. Good correlations between soil water repellency, organic carbon fractions and aggregate stability were found. Under no-till, crop rotations can be altered to increase soil stability by inducing greater water repellency in the soils. The findings suggest that water repellency is a major property influencing soil structure stabilization, thus providing a useful quality indicator
Behrends Kraemer F., P. Hallett, H.J.M. Morrás, L. Garibaldi, D. J. Cosentino, M. Duval, J.A. Galantini. 2019. Soil stabilisation by water repellency under no-till management for soils with contrasting mineralogy and carbon quality. Geoderma 355: 113902. Doi 10.1016/j.geoderma.2019.113902
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Nitrogen balance in a plant-soil system under different cover crop-soybean cropping in Argentina
Cover crops (CC) provide many benets for the soil and the following crop but their effects on nitrogen (N) release and balance in continuous no-tillage soybean (Glycine max L. Merr.) production are little known. Estimation of the biological nitrogen xation (BNF) in intensive agricultural systems under soybean is essential to understanding the N dynamics and to determining the balance and crop demand. This study (2006–2011) was performed on a Typic Argiudoll under no-tillage in the province of Santa Fe, Argentina. The aims were to study the effect of fall winter CC, such as wheat (W), oats (O), oats + vetch (O + V) and vetch (V), on the yield and N-content of the following crop (soybean) and to quantify the contribution of the BNF and N-balance. Three methodologies were used for BNF estimation: 1) a linear regression model between BNF and N-uptake by soy- bean; 2) the natural 15N abundance in soybean and 3) the average BNF in the Pampa region. Gramineous CC developed more dry matter than pure legume species, with intermediate values for the gramineous-legume mixture. Biological xation provides 60–70% of absorbed N, according to the estimation method. Within the rainfall range of 500–1000 mm during the soybean cycle, CC did not affect the grain yield or soybean dry matter production. The partial N-balance was always positive, with differences between the techniques used for BNF estimation. Cover crops have contributed to the positive soil N-balance. Gramineous CC stored 22% more N- content in the soil surface layer than the others. Cover crops showed 15% higher index of N-stratication on the surface compared to the control soil. Using CC would be an efficient alternative to produce biomass and to supply N to the soil for the subsequent crop

Landriscini M.R., J.A. Galantini, M.E. Duval, J.E. Capurro. 2019. Nitrogen balance in a plant-soil system under different cover crop-soybean cropping in Argentina. Applied Soil Ecology 133:124-131. doi:  10.1016/j.apsoil.2018.10.005

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2018 - - - - - - - - - - - - - - - - - - - - - - - - - -



Cover crops in the Southeastern region of Buenos Aires, Argentina: effects on organic matter physical fractions and nutrient availability
In the Southeastern region of Buenos Aires province, soybean monoculture has led to a decline in soil organic matter (SOM) levels in soil, mostly the labile fractions. The reduction of SOM has a negative impact on the soil environment. Cover crops (CC) constitute an alternative to maintain or improve SOM levels. The aim of this study was to determine the effect of oat as CC on (a) the SOM dynamics and (b) the availability of macro- and micronutrients in a representative soil of the Southeastern region of Buenos Aires province. Total organic carbon (TOC) and nitrogen (TON), as well as their labile and mineral-associated fractions, and macro- and micronutrient availability were determined. The treatments were soybean monoculture with and without oat as cover crop. The increases in SOC and TON were 22.7% and 11%, respectively, when CC was included to the soybean monoculture. These increases were observed mostly in the labile fractions, with increases of 61.3 and 38.7% for the particulate coarse organic carbon (PCOg) and particulate fine organic carbon (PCOf), respectively, and 79.2% for the particulate coarse organic nitrogen (NOPg). Regarding the nutrients, an increase of 47.6% was observed in manganese (Mn) in the first 5 cm of soil depth, and a decrease in phosphorous (P) availability in the same soil layer due to its consumption and retention by CC. It can be conclude that CC presented a surface soil effect on the dynamic of SOM, increasing C, N, and available Mn contents, but decreasing soil P availability
Oat, Soybean monoculture, Particulate organic carbon and nitrogen
Beltrán M.J., H. Sainz Rozas, J.A. Galantini, R.I. Romaniuk, P. Barbieri. 2018. Cover crops in the Southeastern region of Buenos Aires, Argentina: effects on organic matter physical fractions and nutrient availability. Environmental Earth Sciences 77:428. DOI: 10.1007/s12665-018-7606-0.
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Labile soil organic carbon for assessing soil quality: influence of management practices and edaphic conditions
Soil organic carbon (SOC) is a complex set of pools, and to understand its dynamics it is necessary to know which of these pools are sensitive to the edaphic and climatic conditions or the agricultural practices, or to both. The objectives of this study were to evaluate the relationships between permanganate oxidizable C (POX-C) and various soil organic carbon fractions in different land-uses and soil types, and to examine whether the POX-C fraction is sensitive to different agricultural management practices in soils under no-tillage. Three treatments were identified at four sites located in the Argentine Pampas region: two different agricultural scenarios in terms of crop rotation, fertilizers and use of agrochemicals (Good Agricultural Practices and Poor Agricultural Practices, GAP and PAP, respectively) and an undisturbed natural (NE) environment adjacent to the agricultural sites as the control treatment. The following organic fractions were quantified: SOC, coarse and fine particulate organic carbon (POCc and POCf, respectively), hot water and acid extractable organic carbon (HWC and HAC, respectively) and POX-C. Soil POC values ranged from 0.46 to 7.29 g kg1, HAC values ranged from 1.50 to 6.73 g kg1, HWC values ranged from 0.20 to 1.10 g kg1 and POX-C values ranged from 0.41 to 1.04 g kg1 soil, POCc being the most variable fraction (CV=72%) and POX-C the least (CV=22%). Soil POCc and POCf at 010 cm, and POCc at 1020 cm were largely explained by management practices with a component of variance >50%. The relationship between POX-C and SOC was generally stronger (R2=0.760.92) than POX-C with other organic fractions and where depth and site factors have a greater influence on this relationship than management practices. Among the labile fractions, the most sensitive indicators of soil quality in agricultural soils were POCf and HWC, which displayed the highest F-statistic values. Despite the dilute solution used (0.02 mol L1 KMnO4) the POX-C demonstrated limited sensitivity to different agricultural practices. However, this methodology could be used to estimate SOC regarding site conditions and depths. The POCf was the fraction most affected by agricultural practices, indicated by high relationships with both the soil physical attributes (macroporosity, bulk density, and density, volume and stability of aggregates) and the agronomic parameters (soybean and maize yields).
Permanganate oxidizable carbón, Particulate organic carbón, Hot water extractable carbón, Acid extractable carbón, Agricultural practices
Duval M.E, J.A. Galantini, J.M. Martínez, F. Limbozzi. 2018. Labile soil organic carbon for assessing soil quality: influence of management practices and edaphic conditions. Catena 171 316-326. DOI 10.1016/j.catena.2018.07.023
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Contribution of nitrogen mineralization indices, labile organic matter and soil properties in predicting nitrogen mineralization in Mollisols

The objectives of this study were i) to measure the nitrogen (N) mineralization potential; ii) to evaluate N mineralization indices and labile soil organic matter (SOM) fractions, and iii) to analyze their interrelationship with soil properties for predicting the potential N mineralization in Mollisols under no-tillage of the Argentine Pampas. Potential N mineralization pool was determined by aerobic incubation of soil samples from 0-20 cm depth at 25°C for six drying and rewetting cycles over 21-24 weeks. The data were fitted to a first-order exponential equation to determine the potentially mineralizable N at a variable (N0) and at a fixed constant rate (N0kf). Several N mineralization indices, labile organic fractions and soil properties were determined. A broad range of values were found for N 0 (29.9-220.7 mg kg-1) and N0kf (26.9-172.4 mg kg-1). A close relationship (P<0.001) was found between N0 and N 0kf with a high coefficient of determination (R2=0.94). High R2 values were found for predicting N0 and N0kf using the principal component as regress variable. Total and labile organic fractions and sand content, explained the majority of the variance. No single N mineralization index, labile organic fraction or soil property can accurately predict the potential N mineralization at a variable and at a fixed constant rate. The potential N mineralization pool may be controlled mainly by the variables related to the size of SOM and some labile fractions, i.e. soil organic C (SOC) and N (SON), soluble carbohydrates (CHs) and fine particulate organic N (fPOM-N) in these Mollisols.

Martínez J.M., J.A. Galantini, M.E. Duval. 2018. Contribution of nitrogen mineralization indices, labile organic matter and soil properties in predicting nitrogen mineralization in Mollisols. Journal of Soil Science and Plant Nutrition. 18 (1) 73-89. 
doi: 10.4067/S0718-95162018005000401
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A rapid method for estimating labile carbon and nitrogen pools in Mollisols under no-tillage

The objective of this study was to adapt the partial chemical digestion method for estimation of labile soil organic matter pools by evaluating the effect of different digestion times in Mollisols of the Argentine Pampas. The soils were sampled from nine agricultural fields under notillage at the 0–20 cm depth. A chemical method was performed through partial soil digestion with dilute sulphuric acid at 100°C on the basis of four digestion times: 1 (Nd1), 2 (Nd2), 4 (Nd4) and 6 (Nd6) hours. Soil organic carbon (C) and nitrogen (N) fractions were determined. The extracted organic N (Nd) ranged from 0.076 g kg-1 to 0.273 g kg-1, with a mean of 0.154 g kg-1. Statistically, the means for each digestion time indicated highly significant differences (P = 0.008). High correlations were found between Nd for different times and labile C and N fractions. However, the best fit prediction was observed between Nd2 and soil total carbohydrates (CHt), with a high coefficient of determination (R2 = 0.94). Partial chemical digestion for 2 h can be used as a rapid indicator to accurately predict CHt. Because of its speed and simplicity, this method may also be useful for rapid soil quality assessments.


Martínez J.M., J.A. Galantini, M.E. Duval. 2018. A rapid method for estimating labile carbon and nitrogen pools in Mollisols under no-tillage. Archives of Agronomy and Soil Science 64 (9) 1321-1327, 
doi: 10.1080/03650340.2017.1422123
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Estimating soil organic carbon in Mollisols and its particle-size fractions by loss-on-ignition in the semiarid and semihumid Argentinean Pampas
Recent interest in soil quality assessment underscores the need for an accurate measurement of soil organic carbon (OC) and its labile fraction, i.e. particulate OC. The loss-on-ignition (LOI) method has been proposed as a rapid, inexpensive and accurate method for estimating OC. The objectives of this study were i) to test the LOI method for soil organic matter (SOM) and particulate organic matter (POM), and ii) to evaluate the use of an adequate conversion factor (CF) for predicting OC from organic matter (OM) in soil and different particle-size fractions in several Mollisols of the Argentinean Pampas. Several agricultural fields (140) under no-tillage were sampled before crop sowing at 0–20 cm. SOM was physically separated by wet sieving, obtaining a coarse particle-size fraction (CPF, 105-2000 μm) and a medium particle-size fraction (MPF, 53–105 μm). Organic C and OM were determined by dry combustion (DC) and by LOI in whole soil (WS) and in both particle-size fractions. The values of OC (0.7–3.6 g 100 g−1) and OM (1.9–7.9 g 100 g−1) varied greatly between the soils. High and significant relationships (P < 0.001) were found between the different soil fractions determined by DC and LOI, with OM (105–2000 μm):OC (105–2000 μm) (R2= 0.94) > OM(WS):OC(WS) (R2 = 0.88) > OM(53–105 μm):OC(53–105 μm) (R2 = 0.82). These results indicated that the C content in each fraction was in the order of 45%, 50% and 41% for WS, CPF and MPF, respectively. The LOI method can predict OC in CPF (105–2000 μm) with reliable accuracy. The CF for predicting OC from OM was variable for each soil fraction considered: 2.23 for WS, 2.00 for CPF and 2.44 for MPF.

Martínez J.M., J.A. Galantini, M.E. Duval, F.M. López, J.O. Iglesias. 2018. Estimating soil organic carbon in Mollisols and its particle-size fractions by loss-on-ignition in the semiarid and semihumid Argentinean Pampas. Geoderma Regional 12: 49-55. 
DOI: 10.1016/j.geodrs.2017.12.004
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Nitrogen mineralization indicators under semi-arid and semi-humid conditions: influence on wheat yield and nitrogen uptake

The objectives were i) to assess indicators for potential nitrogen (N) mineralization and ii) to analyze their relationships for predicting winter wheat (Triticum aestivum L.) growth parameters (yield and N uptake, Nup) in Mollisols of the semi-arid and semi-humid region of the Argentine Pampas. Thirty-six farmer fields were sampled at 0–20 cm. Several N mineralization indicators, wheat grain yield and Nup at physiological maturity stage were assessed. A principal component (PC) analysis was performed using correlated factors to grain yield and Nup. The cluster analysis showed two main groups: high fertility and low fertility soils. In high fertility soils, combining PCs in multiple regression models enhanced the wheat yield and Nup prediction significantly with a high R2 (adj R2 = 0.71–0.83). The main factors that explained the wheat parameters were associated with water availability and N mineralization indicator, but they differ according to soil fertility
Martínez J.M., J.A. Galantini, M.E. Duval, M.R. Landriscini, R.J. García, F. López. 2018. Nitrogen mineralization indicators under semi-arid and semi-humid conditions: influence on wheat yield and nitrogen uptake, Communications in Soil Science and Plant Analysis 49(15) 1907-1921. 
doi: 10.1080/00103624.2018.1485931
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Edaphic forms of phosphorus in no-tillage cropping sequences in the Argentine southern central Pampas
Agriculture significantly affects phosphorus (P) availability, thereby modifying P equilibrium. The objective of this study was to evaluate the effect of different crop sequences on the dynamics of soil P forms under no tillage. Soil samples were collected from a system of five sequences initiated in 1998. The agricultural sequences followed the scheme crop diversity (CD), crop-pasture (CP, without grasses), traditional crops 1 (TC1, for limited soils), traditional crops 2 (TC2), and traditional crops 3. TC3). The available P (Pe) was determined and soil P was fractionated. Organic P extracted with NaOH (PoNa) was analyzed by spectrometry UV–visible and IR. Under CP, the available P decreased in both organic and inorganic labile forms, whereas PoNa values increased. Higher Pe was observed in TC1and TC3. The E4/E6 rates showed that the systems with the lowest degree of aromaticity were CP, TC1 and TC3. These three management sequences presented high PoNa values and, from these values, CP showed lower values for Pe, indicating differences in the chemical quality of the molecule. The 1050/1260 spectra, which relates an aliphatic ester to an aromatic ester, indicated that the CP and TC3 ratios were 1.3 and 0.94, respectively. In other words, the predominant type of ester in CP is aliphatic and in TC3 predominant esters would be of the aromatic type. Crop rotations can produce changes in organic P forms, which will modify the predominant functional group and the way this nutrient interacts with the soil matrix. Inclusion of pastures in a rotation maintains plant-available P at lower levels. However, this is the sequence that keeps moderately labile P (PoNa) at higher levels

Suñer L., R. García, J.A. Galantini, H. Forján, A. Paz González. 2018. Edaphic forms of phosphorus in no-tillage cropping sequences in the Argentine southern central Pampas. Geoderma 323: 107-115. DOI: 10.1016/j.geoderma.2018.02.027
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2017 -  - - - - - - - - - - - - - - - - - - - - - - - - - -


Morpho-structural evaluation of various soils subjected to different use intensity under no-tillage

According to many evidences, in Argentina, no-tillage (NT) coupled with soybean monoculture leads to adverse soil structure features. While some farmers have simplified the production system through soybean monoculture others have intensified the land-use by increasing the number and diversity in the crop sequence. The effects of this intensification, in terms of soil structural quality, are contradictory, possibly caused by the increase of machinery traffic. In order to assess soil structural quality and the performance of selected morpho-structural variables with different levels of intensification, we analyzed plots under NT with high and low crop sequence intensification (Good −GAP- and Poor −PAP-, agricultural practices respectively) and reference plots in four soils (two Argiudolls, an Haplustoll and an Hapludert) of the Argentinian Pampean region. The morpho-structural variables assessed were Visual Evaluation of Soil Structure at field scale (VESS), visible porosity (Vp), roundness (Rd), eccentricity (Ecc) and 3-D aggregate features (faces, corners and edges). Plots with higher frequency of cereals in the sequence (GAP) presented on average higher VESS scores, higher Vp values and less rounded aggregates with more faces and corners, suggesting that crop sequence intensification induces favorable structural features. VESS, Vp, number of faces and corners were strongly correlated with aggregate stability tests mainly with the fast and fast10s test (r: −0.56, −0.74; 0.48, 0.52; 0.46, 0.49 and 0.42, 0.50, respectively) and with the more labile organic carbon fractions −POCc and POCf- (r: −0.49, −0.5; 0.5, ns; 0.38, 0.48 and 0.31, 0.43, respectively). These observations suggest that the variables examined, concerning aggregates and pores were sensitive to changes in crop sequence and are useful soil quality indicators. However, the occurrence of platy structures also under GAP shows the need to adjust the VESS method to the NT system. Besides, the effect of agricultural intensification on soil morphology was modulated by soil type. In consequence, this last factor has also to be considered for the definition of a quality indicator to track the effect of crop sequences intensification under no-till management.

Behrends K.F., M.A. Soria, M.G. Castiglioni, M. Duval, J. Galantini, H. Morrás. 2017. Morpho-structural evaluation of various soils subjected to different use intensity under no-tillage. Soil & Tillage Research 169: 124-137.
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Production and quality of cover crops in soybean monoculture

Cover crops (CC) are an alternative to improve soil organic matter, capture labile nutrients and minimizing its leaching losses during long-term fallows, typical of agricultural systems with high frequency soybean (Glycine max L. Merr.). The objective was to evaluate the production and nutrient content of different CC in simplified system (soybean monoculture) under no-tillage. CC used were: (T) bread wheat (Triticum aestivum L.), (A) oat (Avena sativa L.), (V) vetch (Vicia sativa L.) and (A+V) oat + vetch. Upon drying the CC was determined: production of total aerial dry matter (MS), biochemical composition (cellulose, hemicellulose and lignin), macro- and micronutrients. Dry matter production in T and A ranged between 7.2 and 11.1 Mg ha-1, differing significantly from V with values between 4.1 and 4.6 Mg ha-1. Carbon concentration did not differ between CC (43-45% C). The amount of N accumulated in aboveground biomass ranged between 102 and 212 kg N ha-1, showing differences only between years. The concentration of structural polymers clearly differ between CC species, where T and A showed higher concentrations of cellulose and hemicellulose regarding V. Conversely, V showed higher concentrations of nonstructural carbohydrates and lignin than grasses. Winter grasses as CC were more efficient in producing MS and therefore more effectives to contribute to increased soil organic matter, promoting the recycling of macro- and micronutrients, preventing the leaching of those most labile.
Wheat-vetch, Biochemical composition
Duval, Matías E.; Galantini, Juan A.; Capurro, Julia E.; Beltrán, Marcelo J. 2017. Producción y calidad de diferentes cultivos de cobertura en monocultivo de soja. Ciencias Agronómicas (FCA-UNR) XXIX 7-13.
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Aggregate stability of an Argiustol under different tillage systems in the Southwest of Buenos Aires

Land mismanagement leads to degradation of soil structure, affecting the ecosystem and jeopardizing the productive potential of soils. Tillage systems modify carbon content and distribution, and hence influence the structural stability of soils. This study discusses the effect of no tillage (NT) and conventional tillage (CT) on aggregate-size distribution and carbon content in comparison with an uncultivated or natural soil (Nat).The soil samples were taken to the 0-5 and 5-10 cm depths. They were analyzed for total organic carbon, coarse particulate organic carbon, fine particulate organic carbon and mineralassociated organic carbon. The content of total carbohydrates and soluble carbohydrates was determined. The aggregates were isolated by dry- and wet-sieving and their stability was calculated. All organic carbon forms within the 0-5 cm depth decreased as soil disturbance was higher, whereas no differences were observed in the 5-10 cm depth between NT and CT. The fraction of the dry-sieved aggregates smaller than 1 mm in the 0-5 cm depth turned out to be the most sensitive to tillage; it increased in the order Nat (17.3%)< NT (24.9%)< CT (34.6%), following the same tendency as aggregate stability. Tillage reduction tended to increase the amount of aggregates larger than 2.8 mm, with values of 66 (Nat), 57 (NT) and 37% (CT). Soluble carbohydrate content in the various sizes of the aggregates isolated by dry-sieving followed the same tendency as aggregate stability in aggregates larger than 2.8 mm and smaller than 1 mm. Aggregate stability was associated with the management-sensitive fraction smaller than 1 mm.
Aggregates, Organic carbon, Organic fractions, Soil cementing agents
Iglesias J.O., J.A. Galantini, A. Vallejos. 2017. Estabilidad de agregados de un Argiustol del SO Bonaerense con diferentes sistemas de labranza. Ciencia del Suelo 35(2) 189-204
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Rapid characterisation of agro-industrial effluents for environmental fate by UV–visible and infrared spectroscopy from fractions obtained by centrifugation

Agro-industrial systems (e.g. dairy farms, feed lot, pig breeding and food processing plants) provide large quantity of organic wastes that could be recycled within the productive systems. However, the basic chemical characterisation is not enough to predict the effect that they may generate on the environment. In this study, a centrifugation process was applied at various speeds between 3000 and 15,000 rpm and carried out separately on two different livestock effluents (dairy farm and pig anaerobic digestate), in order to obtain supernatants and precipitates, which were studied separately. The more water soluble fractions, with lighter components and/or simpler structures, remained as liquid supernatants, while the more complex fractions, with higher molecular weight and/or water insoluble fractions, constituted the solid precipitates. An increase in the centrifugation rate did not produce the differential precipitation of dissimilar functional groups. Hence, 5000 rpm was the most adequate velocity since it generated clear supernatants without denaturation of the organic matter. A basic cost-effective chemical analysis, complemented with ultraviolet-visible and Fourier transform infrared spectroscopy, enables a set of properties to be established qualitatively and quickly for the multiple components of the organic matter for its later use as fertilisers or amendments. This rapid and economical technique allows for a characterisation prior to the reuse of the effluents, which is necessary to optimise their application and avoid environmental problems.

Iocoli G.A., O.I. Pieroni, M.A. Gómez, M.B. Alvarez & J.A. Galantini. 2017. Rapid characterisation of agro-industrial effluents for environmental fate by UV–visible and infrared spectroscopy from fractions obtained by centrifugation. International Journal of Environmental Analytical Chemistry 1-12 pags. DOI: 10.1080/03067319.2017.1354993
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A rapid chemical method for estimating potentially mineralizable and particulate organic nitrogen in Mollisols

The objective of this study was to obtain an indicator of labile nitrogen (N) through a cost- and time-saving procedure by evaluating the relationships among potentially mineralizable N (N0), particulate organic matter N (POM-N) and soil organic N extracted through partial soil digestion with different concentrations of sulfuric acid (H2SO4). Soil sampling (0–20 cm) was from nine fields under no-tillage. The N0 and POM-N were determined by long-term aerobic incubation and soil physical fractionation, respectively. A simple chemical method was developed by soil digestion at 100°C for 4 h with different concentrations of H2SO4 (0.1, 0.5, 1, 6, 12, and 24 mol L−1). All acid concentrations showed significant relationships with N0 as POM-N; however, the best prediction was resulted for 0.5 mol L−1 (R2 = 0.90–0.94, respectively), thus using this methodology as soil labile N indicator. This method would optimize N0 and POM-N estimation in short term and at a low cost.
Palabras clave
Martínez J.M., J.A. Galantini. 2017. A rapid chemical method for estimating potentially mineralizable and particulate organic nitrogen in Mollisols. Communications in Soil Science and Plant Analysis 48(1) 113-123.
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Tillage effects on labile pools of soil organic nitrogen in a semi-humid climate of Argentina: A long-term field study

Tillage systems strongly affect nitrogen (N) mineralization. However, there is still only limited information on the relationship between N in labile soil organic matter (SOM) fractions and crop N uptake under different tillage systems in areas with poor water availability. This study discusses the long-term effect of two tillage systems on i) the N-content in labile organic matter fractions and their relationship with the N mineralization potential at three depths (0-5; 0-10 and 0-20 cm), ii) the factors that affect the N mineralization potential, and iii) the relationship between potentially mineralizable N (N0) and crop N uptake in a semi-humid climate. In a long-term experiment, a Typic Argiudoll was sampled under two contrasting tillage systems: no-tillage (NT) and conventional tillage (CT). The soil sampling was performed over four years of the crop sequence (2003, 2009, 2010 and 2011) when the plots were sown with winter wheat (Triticum aestivum L.). They were analyzed for N0 in the form of anaerobic N, soil organic nitrogen (SON), physically separated SOM fractions and crop N uptake. Higher values of SON and labile soil N fractions were observed under NT at all three depths. Significant differences in N0 were found between the tillage systems, with greater values under NT. Significant (P <0.05) and positive correlations between N0 and fine particulate organic carbon (fPOM-C) (r≥0.66) were found in CT and in NT at the three depths, whereas highly significant (P <0.001) and negative relationships between N0 and fine particulate organic N (fPOM-N) (r≥-0.83) were found under both tillage systems at 0-5 and 0-10 cm. The most pronounced difference in these relationships between tillage systems was observed at the 0-5 cm soil depth. Significant correlations of N0 with residue input from previous crops and the fallow period were observed under both tillage systems and for all three depths. Regarding the relationships between N0 and wheat N uptake, no significant correlations were found for any tillage system or depth. Soil organic N fractions were shown to be strongly influenced by the residue input from the previous crop and by variable weather conditions during the fallow period. The higher content of SON fractions under NT was associated with a higher N mineralization potential, however, it did not result in increased N availability and N uptake by wheat, because of climatic conditions during the crop growing season.
Palabras clave
Martínez J.M., J.A. Galantini., M. Duval. 2017. Tillage effects on labile pools of soil organic nitrogen in a semi-humid climate of Argentina: A long-term field study. Soil & Tillage Research 169:71-80
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Adjustments in organic carbon estimation by loss/on/ignition method in Mollisols of the Southwest Buenos Aires Province

Soil organic carbon (SOC) quantification is crucial for understanding the cycling in the terrestrial ecosystems. It is important  to use methodologies of easily implementation that determine the SOC properly. The aims of this study were to determine: i) the optimal temperature and time to maximize the prediction of SOC and ii) a suitable conversion factor (fc) to estimate the SOC by loss-on-ignition method (MC) in Mollisols of the southwest Buenos Aires Province (SOB). During 2010 and 2011, seventy plots under no-tillage located in the SOB, Argentina, were sampled at 0-20 cm depth. Soil organic carbon values determined by dry combustión (CS) in an elemental analyzer were considered as reference values. Soil organic matter (OM) values were determined by MC in a muffle furnace at different temperatures (360, 430, 550 and 600 °C) and times (during 2 and 4 hours). No significant interactions were detected between both factors. Significant effect of different temperatures (p<0.001), while, no significant  effect of calcination time (p=0.66) were detected. Linear regressions between SOC and OM at different temperatures showed significant relationships (p <0.001) with high coefficients of determination (R2 =0.91-0.95). The best prediction of SOC was found with 550°C temperature (R2= 0.95). The fc was 2.2, which would indicate that in these soils the C content in OM was 45%. These results suggest that MC with an adequate fc, is an easily implementable protocol that provides estimation of SOC comparable with the reference CS method.
Soil organic matter, dry combustion, conversion factor.
Martínez Juan Manuel, Matías E. Duval, Fernando M. López, Julio O. Iglesias, Juan A. Galantini. 2017. Ajustes en la estimación de carbono orgánico por el método de calcinación en molisoles del sudoeste bonaerense. Ciencia del Suelo 35(1) 181-187.
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Links
https://drive.google.com/open?id=1o3oaXtsLU4809cp399Nn4UIFFHeWCtAd



Use of a three-compartment model to evaluate the dynamics of cover crop residues

Cover crop (CC) residues protect the soil from erosion and their permanence on the surface is largely influenced by their biochemical constituents. In this study the dynamics of CC residue decomposition by applying mathematical models was described. The kinetics of decomposition of residues was obtained from a laboratory incubation experiment. Three CC shoot residues were applied on the soil surface and incubated for 362-days (with eight sampling times). Oats and vetch residues decomposed the most than clover, which k values were 3.6 × 10³, 3.7 × 10³ and 5.3 × 10³ day¹, respectively. The three-compartment model (non-structural carbohydrates, cellulose-hemicellulose and lignin) to simulate residue decomposition, presented a close fit between simulated and measured data. The decomposition rate constant (k) of CC can be used to estimate how long residues will remain in the field and how they could affect soil organic carbon.
Palabras clave
Sa Pereira E, J.A. Galantini, M. Duval. 2017. Use of a three-compartment model to evaluate the dynamics of cover crop residues. Archives of Agronomy and Soil Science 63(11)1623-1629. http://dx.doi.org/10.1080/03650340.2017.1296137
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Decomposition from legume and non-legume crop residues: effects on soil organic carbon fractions under controlled conditions

Cover crop (CC) residues protect the soil from erosion and their permanence on the surface is largely influenced by their biochemical constituents. We performed a study under controlled conditions to investigate the dynamics of legume and non-legume CC residues decomposition and the transformations of the soil labile organic carbon fractions in the surface layer (0-15 cm). The experiment was carried out on a Typic Argiudoll (clay loam, 27.4 g kg¹ soil organic matter, 14 mg kg¹ extractable phosphorus and 6.5 pH) placed in undisturbed pots (1570 cm³) in a greenhouse under controlled conditions of temperature (25 ± 1 °C). We evaluated three CC species (oat, Avena sativa L.; vetch, Vicia sativa L.; Persian clover, Trifolium resupinatum L.) and a no-CC control (fallow). Shoot residues were applied on the soil surface at 5.4, 5.4 and 2.7 g dry matter (equivalent to 6, 6 and 3 Mg ha¹ for oat, vetch and clover, respectively) and incubated for 362-days (eight sampling times). The water content in the pots was maintained periodically by weight at 60% of soil water-holding capacity. The soil samples were analyzed for particulate organic carbon (POC), and total and soluble carbohydrates (CHt and CHs, respectively). Oat and vetch residues decomposed faster than clover, with the decomposition rate constant (k) values of 1.3, 1.4 and 1.9 year¹, respectively. At the end of the experiment, POC concentration was lower in vetch (1.83 g kg¹) and clover (1.96 g kg¹) than in oat (2.21 g kg¹) and fallow (3.00 g kg¹), indicating a loss of 45-64% from their initial values. Soil CHt was influenced by residue quality, where the periods of greatest residue decay (vetch 21-59 days and oat 93-130 days) corresponded to higher soil CHt. Hence, this organic carbon fraction is sensitive to residue decomposition and can be indicators of changes in soil organic matter over short periods of time.
Palabras clave
Sa Pereira E, M. Duval, J.A. Galantini. 2017. Decomposition from legume and non-legume crop residues: effects on soil organic carbon fractions under controlled conditions. Spanish Journal of Soil Science 7(2) 86-96 https://doi.org/10.3232/SJSS.2017.V7.N2.06
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Quality of cover crops in Southwest Bonaerense under no tillage systems

No-tillage system with fall/winter cover crop (CC) ensures that large amounts of different types of organic residue are supplied to the soil. The biochemical composition of each residue, especially its C:N ratio and lignin, plays an important role in nitrogen (N) and carbon (C) soil dynamics, as well as the availability of nutrients for the next crop. The objective of this study was to evaluate the quality parameters of CC based on the quantity and quality of the residue contributed. The experiments were carried out on Argiudioles typical of the Coronel Suárez (Pcia. de Bs. As.) Species used as cover crops were: Oats (Avena sativa L.), Hairy vetch (Vicia sativa L. Thell.), Clover persia ‘‘grazing’’ (Trifolium resupinatum L var. ‘‘Lightning’’), Clover Persia ‘‘coverage’’ (Trifolium resupinatum L. var. ‘‘Laser’’). The determinations were: a) Production of forage (Mg MS ha-1), b) N, c) neutral detergent fiber (NDF) and acid (FDA), nonstructural carbohydrates (CNES), carbon (C%) and Lignin. The aerial biomass of Hairy vetch presented the highest concentrations of N in the dry matter (MS) produced. When the oats were fertilized they were able to increase the contributions of N to the soil present in the aerial biomass of the residue to 50%. The aerial biomass of Hairy vetch presented the highest yields and concentrations of lignin. The different CC presented C:N and LIG:N ratios that were based on the amount of MS produced by the aerial biomass of the crop and its phonological state at the time of drying. The temperature and the moment of evaluation modified quantity and quality of the residues. The parameters evaluated were sensitive to these changes.
Coverage crops, cellulose, hemicellulose, lignin.
Sá Pereira E., J.A. Galantini, A. Quiroga. 2017. Calidad de cultivos de cobertura en sistemas de siembra directa del sudoeste bonaerense. Ciencia del Suelo 35(2) 337-350
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Changes in soil pH and phosphorus availability during decomposition of cover crop residues

The aim of this study was to determine the effect of winter cover crops (CC) residues on soil pH and phosphorus (P) availability. Three incubation assays were performed in pots using two CC: vetch (V) (Vicia villosa Roth.) and oats (Oa) (Avena sativa L.). Soil samples were taken from ten sites at 0-20 cm depth. The rate of residues were 0 (D0), 10 (D1), 20 (D2), 30 (D3) and 40 (D4) g dry matter kg-1 soil and the soil sampling was after 10, 20, 30, 60, 90 and 120 days of incubation. Soil pH, extractable P (Pe), and soil organic matter (SOM) and its fractions were determined. The pH increase was correlated with the rate applied (D1< D2< D3< D4). No differences were found for pH comparing V and Oa residues with low residue rates. Soil pH changes were dependent from initial pH and SOM fractions in different soils across the incubation period. The multiple regression models showed that the pH changes were dependent on initial pH level and SOM fractions with a high R2 (0.81). Cover crops residues and its quantities produced different changes on pH - especially at the beginning of the incubation- which influenced the P availability.

Vanzolini J.I., J.A. Galantini, J.M. Martínez, L. Suñer. 2017. Changes in soil pH and phosphorus availability during decomposition of cover crop residues. Archives of Agronomy and Soil Science 63 (13) 1864-1874. http://dx.doi.org/10.1080/03650340.2017.1308493
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Soil organic matter quality, macro and micronutrient availability in response to the inclusion of wheat as cover crop
Cover crops appear as an option to improve the balance of carbon (C) and its quality. The aim of this study was to study the effect of wheat as a cover crop on the dynamics of organic matter and the availability of some macro and micro nutrients under different crop rotations. This work was carried out in a long-term experiment under non-tillage system. Treatments were: soybean-soybean (S-S), soybean-cover crop-soybean (S-CC-S), corn-soybean-wheat/soybean (C-S-W/S) and corncover crop-soybean-wheat/soybean (C-CC-S-W/S). As principal results, it was observed that the fraction of organic carbón between 53 and 105 mm (COPf) was significantly increased when CC were added to soybean monoculture. The carbón fraction of less than 53 mm (COa), was higher when cover crop were incorporated to the rotations. Soybean monoculture had a value of total organic carbon (COPg + COPf) of 11.1 g kg-1, being the lowest value of all treatments and significantly lower than S-CC-S (17 g kg-1). Soil macronutrients concentration had no differences between treatments. Rotations with grasses and legumes (M-S-T/S) determined in general a greater accumulation of zinc (Zn) and manganese (Mn) (2.2 and 63 mg kg-1 respectively) than soybean monoculture (1.4 and 50 mg kg-1) in the depth of 0-5 cm. The concentration of these micronutrients was positively correlated with organic matter content. In this year of study, the CC did not seem to have affected the concentration of micronutrients, thus successive analysis should be performed in order to study the effect of CC in micronutrients dynamics in time.
Organic fractions, nutrient availability, crop rotations
Beltrán M., L. Brutti, R. Romaniuk, S. Bacigaluppo, F. Salvagiotti, H. Sainz-Rosa, J.A. Galantini. 2016. Efecto del trigo como cultivo de cobertura sobre la dinámica de la materia orgánica en el suelo y la disponibilidad de macro y micronutrientes. Ciencia del Suelo 34(1) 67-79.
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Comparison between agricultural and natural quality indices based on organic carbon
Literature exhibit a great number of soil quality indices, many of them based on organic carbon and its fractions, for a wide variety of natural and cultivated soils. In four sites located in the argentine Pampa region, the effect of the sequence and intensity of crop rotations on different organic carbon fractions in no-tilled soils, and the sensitivity of some quality índices to management practices were evaluated. At each site, three treatments were identified: two different agricultural scenarios in terms of crop rotation, fertilizers and use of agrochemicals (diversified intensive and regional representative managements, MID and MRR, respectively) and an undisturbed environment, natural (ASD) adjacent to agricultural sites as control treatment. Agricultural management with a high frequency or soybean monoculture caused COT decreases of about 30% in 0-10 cm depth. These decreases mainly affected labile fractions (particulate CO 105-2000 μm and particulate CO 53-105 μm) which decreased by 50% and 40% with respect an ASD field. These situations present the worst values in the most indices including labile fractions. However, the magnitude of the observed changes in these indices were lower in relation to those associated with the COT. The COT stratification index (0-5:5-20 cm) showed differences between treatments, which ranged from 2.0 (ASD) to 1.5 (MRR). The differential carbon input to the soil between treatments was reflected in the carbon pool index (IRC), considering a simple index to measure and sensitive to detect differences between agricultural managements. The results highlight the importance of the COT as universal indicator and the need to take into account local issues either management and/or seasonal, for the interpretation of the indices associated with the most labile fractions.
Stratification index; soybean frequency; labile fractions.
Duval M.E.; J.A. Galantini; J.M. Martínez; J.O. Iglesias. 2016. Comparación de índices de calidad de suelos agrícolas y naturales basados en el carbono orgánico. Ciencia del Suelo 34(2) 197-209.
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Sensitivity of different soil quality indicators to assess sustainable land management: Influence of site features and seasonality
The turnover rate of labile organic fractions varies continuously due to different soil uses and managements, weather conditions and sampling time. The aim of this study was to quantify the effect of different agricultural management, season and soil type on soil organic carbon (SOC) and its different fractions. The study was conducted on four sites located in the Argentinean Pampas. In each site, three treatments were defined: Good Agricultural Practices (GAP), Poor Agricultural Practices (PAP) and Natural Environment (NE). During two consecutive years (2010 and 2011) and at two different times (February and September) undisturbed soil samples were taken at 0–20 cm depth. Variables assessed included: SOC and its organic fractions: coarse (POCc) and fine (POCf) particulate organic carbon, SOC associated with a mineral fraction (MOC), total (CHt) and soluble (CHs) carbohydrates, bulk density (BD), and large pores (P>30). Also, indices associated with soil and management variables were determined. SOC reductions caused by agricultural practices were mainly from POCc. This fraction represented 34–52% and 50–74% for PAP and GAP, respectively, of the observed in NE. The carbon pool index (CPI) shows that agricultural treatments induced greater variations in all the labile organic fractions compared with SOC and MOC. In turn, the magnitude of variability was different among fractions, where temporal fluctuations increased according to the following order MOC < SOC < POCf <  CHt < CHs < POCc. Independently of the soil type, the CPI was a sensitive indicator of soil quality in these systems under no-tillage. The multivariate analysis has proven to be an efficient analytical methodology for the identification of soil indicators that respond to agricultural practices, in which chemical properties (POCf and CHt), physical (BD and P>30), and indices (SOC: clay, structural index and intensification sequence index) were the variables that best explained the total variance of information of the four sites. Therefore, these indicators/indices should be included in any minimum data set for evaluating the agricultural soil quality under no-tillage in the studied area.
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Winter cover crops in soybean monoculture: Effects on soil organic carbon and its fractions
The current agricultural production systems in the Pampas Region have been significantly simplified by cultivating large land areas under no tillage (NT), where soybean is the predominant crop. These systems with long periods of fall-winter fallow and poor annual input of carbon (C) into the soil lead to soil degradation, thereby affecting physical and chemical properties. A 6-year cover crop study was carried out on a Typic Argiudoll under NT in the south of Santa Fe, Argentina. Various winter species were used as cover crops: wheat (W), oat (O), vetch (V), an oat + vetch mixture (O + V) and a control (Ct) treatment without a cover crop. We examined the influence of cover crops on the following soil organic C-fractions: coarse particulate organic carbon (POCc), fine particulate organic carbon (POCf) and mineral-associated organic carbon (MOC) from 2008 to 2011. Aboveground carbon input by the cover crops was related to the June to October rainfalls. In general, the W and O treatments supplied a higher amount of C to the soil; these gramineous species produced 22 and 86% more biomass than O + V and V. The water cost of including cover crops ranged from 13 to 93 mm compared with Ct. However, this water-use did not affect soybean yields. On average, gramineous species (pure stand or mixture) supplied more than 3.0 Mg C ha-1 year-1 to the soil, whereas V supplied less than 2.0 Mg C ha-1 year-1. Increase in the mean annual C-input by residues into the soil (cover crop + soybean) explained most SOC variation (R2 = 0.61; p < 0.05). This relationship was more evident with labile soil organic fractions, both for POCc (R2 = 0.91; p < 0.001) and POCc + POCf (R2 = 0.81; p < 0.001). The stratification ratios of SOC (SI, 0-5: 10-20 cm) reflected differences among treatments, where >2.0 for W; 1.7 for O, O + V and V, and <1.5 for Ct. Soil physical fractionation by particle size showed that cover crops affected the most dynamic fraction directly associated with residue input (POCc) at 0-5 and 5-10 cm. At 0-5 cm, the effects were observed in the most transformed fractions (MOC and POCf) 4 years after the experiment started, whereas at 0-20 cm, differences in the labile fractions (POCc and POCf) were found at the end of the experiment (6 years). Although C-input by the cover crops fueled decomposition of labile soil organic fractions, concentration of surface SOC and its associated fractions (POCc, POCf and MOC) was modified after 6 years. This effect became noticeable during the third year when the plots under cover crops showed a higher SI than the traditional fallow.
Organic fractions, Carbon balance, carbon stratification index
Duval M., J.A. Galantini, Julia E. Capurro, J.M. Martinez, F.M. López. 2016. Winter cover crops in soybean monoculture: effects on soil organic carbon and its fractions. Soil & Tillage Research 161:95-105 doi:10.1016/j.still.2016.04.006
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Quality and Quantity of Organic Fractions as Affected by Soil Depth in an Argiudoll under Till and No-till Systems
Aims: The aim of this study was to evaluate the long-term effect of tillage systems on the quantity and quality of organic carbon fractions at different soil layers.
Study Design: The experimental design was a split plot with three blocks. The long-term effects (25 years) of conventional- (CT) and no-tillage (NT) systems on a Tipic Argiudoll was sampled at 0-5, 5-10, 10-15 and 15-20 cm soil depth.
Place and Duration of Study: The field experiment was carried out at Tornquist (38° 07’ 06” S - 62°02’ 17” O) and soil sampling was performed during wheat seeding (June 2011).
Methodology: Total soil organic carbon (SOC) content and the following fractions were determined: Coarse particulate (POCc, 105-2000 µm), fine particulate (POCf, 53-105 µm) and mineral-associated (MOC, 0-53 µm) carbon fractions; humic (HA) and fulvic (FA) acids; and total (CHt) and soluble (CHs) carbohydrates. The main physico-chemical properties of HA and FA were analyzed using both FT-IR and fluorescence spectroscopies.
Results: After 25 years, total SOC at the 0-20 cm depth was 9% higher in no-tilled than in tilled soils. The POCf was the SOM fraction that turned out to be the most sensitive to tillage effects. The POCc:POCf:MOC ratio at 0-20 cm was similar for NT (3:14:82) and CT (5:10:84); however, differences were found across soil depths. Tilled soils showed higher aromaticity, starting by CH-degradation, in more superficial soil layers.
Conclusion: The no-tillage system presented a different pattern which can be related to distribution of crop residues and conditions for humification along the soil depth.
Tillage system; soil organic carbon; chemical and physical fractionation.
Galantini, J.A.; M. Duval; J.M. Martinez; V. Mora; R. Baigorri & J.M. García-Mina. 2016. Quality and quantity of organic fractions as affected by soil depth in an argiudoll under till and no-till systems. International Journal of Plant & Soil Science 10 (5) - doi:10.9734/IJPSS/2016/25205
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Nitrogen recovery efficiency by the difference and isotopic dilution methods
The nitrogen (N) use efficiency can be determined by the isotopic dilution or by the difference method. The objectives were to measure the destination of foliar fertilizer and to compare the Nitrogen Recovery Efficiencies (NRE) between the mentioned methods. A field experiment was conducted in the southwest of the Buenos Aires Province (Las Oscuras). Nitrogen rates of 0, and 50 kg N ha-1 were applied at sowing and 0 and 25 kg N ha-1 at anthesis. The enriched urea was applied in anthesis and post-anthesis in the microplots. Yields were low with no response to the initial or foliar fertilization: 2100 and 2154 kg ha-1 for 0 and 50N without foliar N and 2160 and 2303 kg ha-1 with foliar N application. In the isotopic plots, wheat accumulated 83 kg in aboveground biomass and 52 kg N ha-1 in grain without a significant impact of initial or foliar fertilization. Nitrogen from the foliar fertilizer was accumulated 20% in aerial dry matter (ADM) and 12% in the grain, with a recovery of 6 kg N ha-1. Accumulated N in straw ranged 10 kg N ha-1 and unrecovered N varied from 7.2 to 8.3 kg N ha-1 (30%). The NRE varied from 30% to 54 according to the difference method for the initial 0 and 50N treatments in ADM and grain, with an accumulation of 2.4 to 6 kg N ha-1. Isotopic determined NRE was of 70% in ADM and 25 % in grain. Again, grain accumulation was of 6 kg N ha-1. The differences between the methods suggested the need to identify the factors that influence these estimates.
Wheat, 15N, Nitrogen recovery, Foliar urea.
Landriscini M.R., J.A. Galantini, J.M. Martínez. 2016. Destino y recuperación del fertilizante foliar en trigo usando la técnica isotópica de 15N. Ciencia del Suelo 34(1) 155-162.
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Nitrogen fertilization in wheat in the Subhumid Region: nitrogen and water use efficiencies
Water and nitrogen (N) are typically the main wheat crop production factors in regions with water constraints. For this reason, it is of great importance to increase the N use efficiency (EUN) -when applying fertilizers- and the water use efficiency (EUA) in sub-humid environments. The objectives of this study were i) to analyze wheat response to different N rates, ii) to evaluate the EUN and EUA of total aboveground biomass (MST) and grain yield, and iii) to predict these efficiencies with models using principal components (CP) related to MST and grain yield as variables for five years in a sub-humid environment. During the 2008-2012 years, nitrogen fertilization trials were conducted in wheat using four rates (0-25-50-100 kg ha-1) applied at sowing. At physiological maturity, MST and grain yield were measured, and EUN and EUA were calculated for both parameters. The CP analysis was performed using available N and soil moisture at planting, and rainfall in function of MST and grain yield. Three CP were made for each parameter, with a full explanation of 90% and 96% of the MST and grain yield total variance. The NUE predictions used three CP with a high fit (R2 = 0.78 - 0.81) for MST and grain yield. However, the EUA predictions of MST and yield were low (R2 = 0.38 to 0.45), excluding from the model the CP highly influenced by the available N. The high variability in water availability did not allow a significant increase in the EUA by N application, showing that water is the most limiting factor in this environment. The use of CP as regressor variables allowed accurate models for EUN prediction of MST and grain yield.
Triticum aestivum L.; Erratic rainfalls; Principal components.
Martínez J.M., J.A. Galantini, M.R. Landriscini, F.M. López, M.E. Duval. 2016. Fertilización nitrogenada en la región subhúmeda Bonaerense: Eficiencia del uso del agua y del nitrógeno. Ciencia del Suelo 34(1) 81-92.
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Diagnosis of nitrogen fertilization using a chlorophyll meter in wheat under rainfed conditions in the southwest of Buenos Aires province under two different previous crops
The chlorophyll meter could be used to improve the diagnosis of nitrogen (N) deficiency. The objectives of this study were to: i) evaluate wheat (Triticumaestivum L.) yield after two previous crops, ii) assess the effect of the previous crop on the green index (IV) by chlorophyll meter readings, and iii) verify the usefulness of the chlorophyll meter in advanced growth stages of wheat to predict the effect of foliar N application on wheat production and quality in the semiarid region of the southwest of Buenos Aires province. In 2011, a fertilization trial on wheat (cultivar Buck Malevo) was conducted under no-tillage after two contrasting previous crops: wheat and pea (Pisum sativum L.). In each case, different N rates (0, 25, 50, and 100 kg N ha-1) in the form of urea (46-0-0) were applied manually at crop emergence. At anthesis, chlorophyll meter readings were taken in the last expanded leaf and in one half of each plot a foliar N fertilizer was applied at a rate of 25 kg N ha-1. At physiological maturity, total aerial biomass (MST), grain yield, crop N uptake, and protein content were determined. Pea as a previous crop increased the production of MST, grain yield and N uptake, but no effect was detected on protein. Protein content was significantly increased by foliar N fertilizer application, regardless of previous crop. The chlorophyll meter is a useful tool that could be used in advanced stages of wheat to fine-tune the diagnosis of N needs. which would allow improving grain quality in the semiarid environment studied.
Key words Foliar N application; previous crop; green index.
Martínez J.M., M.R. Landriscini, G.V. Minoldo, J.A. Galantini. 2016. Uso de un clorofilómetro para el diagnóstico de la fertilización nitrogenada en la región del sudoeste bonaerense en trigo de secano sobre dos antecesores. Ciencias Agronómicas (FCA-UNR) 28: 35-43.
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Soil porosity in Ustolls under no-till systems in Southwestern Buenos Aires
In agricultural production the appropriate soil physical quality is essential to achieve good crop yields with minor environmental effect. Since no tillage (NT) use broadly expanded in southwestern Buenos Aires, a more detailed knowledge of the physical soil condition under this system is needed. With the aim of analyzing the present physical quality, pore size distribution was determined in Ustolls of southwestern Buenos Aires province under NT (AG) and in quasi pristine environments (AN). Also, the influence of the different granulometric fractions on physical properties was evaluated. Analyzed soils showed a poor subsurface aeration porosity tendency demonstrated by the low macroporosity of the AN (16.6%). In agricultural soils these limitations increased due to lower macroporosity (11.8%). Near 88% of AG presented subsurface physical characteristics that would limit wheat (Triticum aestivum L.) and barley (Hordeum vulgare L.) development, even after 12 years under NT. The main limitation was associated with a low macroporosity (pores> 30 μm) that could affect soil aeration and root growth. Although it is not possible to attribute the macroporosity loss to NT management, the way it has been implemented in the region has not been able to reverse the soil physical quality problems. It is necessary to develop land management practices that contribute to the biotic soil structure regeneration (e.g. rotations with pastures, inclusion of crops with deep roots) to ensure adequate physical fertility in subsurface layers of Ustolls under NT in southwestern Buenos Aires.
Key words. Soil physics; Macropores; Tillage.
López F.M.; M.E. Duval, J.M. Martínez, J.A. Galantini. 2016. Porosidad de Ustoles bajo siembra directa en el Sudoeste Bonaerense. Ciencia del Suelo 34(2) 173-184.
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Soil Humic and Fulvic Acids from Different Land-Use Systems Evaluated By E4/E6 Ratios
Changes in land-use systems such as the introduction of trees on agricultural land can give rise to changes in the physicochemical properties of the soils, also affecting the quantity and quality of organic matter incorporated into the system. The present study assesses humic substances (HS) in the soil such as humic acid (HA) and fulvic acid (FA) by looking at the relationship between the optic densities determined at 465 and 665 nm (E4/E6 ratio). Topsoil samples (0–20 cm) from pine woodlands 60 years of age were compared with agricultural soils of similar age in the central-south region of the Province of Buenos Aires, Argentina. The pH of the topsoil from beneath the pine trees was highly acidic (5.0 vs. 6.2) and a significant increase in the level of organic carbon (OC) was registered. The carbon to nitrogen (C/N) ratio was also higher (by one order of magnitude) beneath the pine trees, although the humification conditions of the soil organic matter (SOM) were good in the soils of both studied land-use systems. The E4/E6 ratio was higher in the HA and FA 2º (second extraction) beneath the pines, indicating a smaller molecular size of the HS bound to the clay minerals. This fact can be attributed to the higher concentration of hydrogen ions beneath the pines and consequently the loss of polyvalent cations, mainly calcium. The most labile organic molecules (FA 1º – first extraction) were of a larger size in soils beneath the pines, most likely owing to a specific characteristic of the Pinus genus, although the fraction in question constitutes a minority fraction among the HS. Clear differences were established between the E4/E6 ratios in HA and FA, making this a highly useful method for determining molecular changes in HS as a result of changes in land use.
Fulvic acid; humic acid; pinus afforestation
Zalba P., N.M. Amiotti, J.A. Galantini, S. Pistola. 2016. Soil humic and fulvic acids from different land use systems evaluated by E4/E6 ratios. Communications in Soil Science and Plant Analysis 47 (13-14) 1675-1679. Doi 10.1080/00103624.2016.1206558
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Effect of sorghum residues on wheat productivity in semi-arid Argentina. I. Stover decomposition and N distribution in crop
Humification and residual effects of 3-month-fallowed sorghum residues on the productivity of winter wheat in a petrocalcic Haplustoll soil in semi-arid Argentina were studied. 15N-labelled urea was applied at sorghum harvesting-mulching (or beginning of fallow, F), wheat seeding (S) and tillering (T). Sorghum residues interacted strongly with N added to the soil. Total aerial plant dry matter (TADM) was lowest (4.94 Mg ha-1) when N was added at the beginning of fallow thus indicating a strong N immobilization, and highest (8.30 Mg ha-1) when N was incorporated at S. The N uptake followed a similar pattern. The percentage of N derived from fertilizer and that of recovery as well as the N content of the TADM increased from F to S to T. These data suggest that the sorghum residues immobilize larger amounts of N when it is applied at the beginning of fallowing than when it is incorporated at a later time such as seeding and tillering.

Rosell R.A., J.A. Galantini, J.O. Iglesias, R. Miranda. 1992. Effect of sorghum residues on wheat productivity in semi-arid Argentina. I. Stover decomposition and N distribution in crop. The Science of the Total Environment, 117/118: 253-261.
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